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Dr. Wenxin Qin
Professor and Head Appointed: 2004
Cancer Research Group
National Laboratory for Oncogenes and Related Genes
Shanghai Cancer Institute
Shanghai Jiao Tong University
Mailing Address
Shanghai Cancer Institute
No. 25/Ln 2200 Xie-Tu Road
Shanghai 200032
China
Contact Information
Phone：+86-21-64436581
Fax：+86-21-64432142
E-mail： wqin@shsci.org
Qualifications
Visiting Scientist, Leroy Hood Laboratory, Institute for Systems Biology, Seattle, USA, 2007-2008
Ph. D., Shanghai Medical College, Fudan University, Shanghai, China, 1998
M. Sc., School of Medicine, Soochow University, Suzhou, China, 1994
B. Sc. College of Life Sciences, Wuhan University, Wuhan, China, 1985
Research Interests
Our group is interested in exploring molecular mechanisms of human hepatocellular carcinoma (HCC) and research areas are involved in the following: functional genomics of cancer, tumor microenvironment and cancer metastasis, biomarker, drug discovery, etc.
In functional genomics of cancer, we identified several novel genes involved in hepatocarcinogenesis by the Marathon-RACE (Marathon-Rapid Amplification of cDNA Ends) or the SMART-RACE (Switching Mechanism at the 5’ end of the RNA Transcript-RACE) method. We designated these novel genes as BNIPL, LASS2, ANGPTL4, ASB8, and C17orf25, respectively. The detailed and latest information for these novel genes can be found on the HUGO Gene Nomenclature Committee (HGNC) web site (http://www.genenames.org) and in our publications.
In the field of cancer metastasis, we focus on the role of tumor acidic-microenvironment in cancer invasion and metastasis. One of our studies in this area was published recently (Cancer Res 2005, Cancer Res 2007). The articles reveal the relationship between vacuolar H+-ATPases (V-ATPases) and cancer metastasis in HCC. V-ATPases have been proposed to have an important role in tumor metastasis, because they play an essential role in maintaining neural cytosolic pH, very acidic luminal pH, and acidic extracellular pH. Our findings firstly report that the inhibition of V-ATPase function via knockdown of ATP6L (a key subunit of V-ATPase complex) expression using RNA interfering technology can effectively retard the cancer growth and suppress the cancer metastasis by the decrease of proton extrusion and the down-regulation of gelatinase activity. Therefore, our results implicate the potential for cancer treatment using V-ATPase as a candidate target.
Using systems biology approach, our group is currently involved in projects discovering biomarkers of diagnosis and prognosis for HCC and drug targets for cancer treatment.
Area of Expertise
Cancer/Hepatocellular Carcinoma, Functional Genomics of Cancer, Tumor Microenvironment, Cancer Metastasis, Biomarker, Drug Discovery.
Selected Publications
1、Xie L, Qin W, Li J, He X, Zhang H, Yao G, Shu H, Yao M, Wan D, Gu J. BNIPL-2 promotes the invasion and metastasis of human hepatocellular carcinoma cells. Oncol Rep. 2007 Mar;17(3):605-10 (Corresponding Author).
2、Lu X, Qin W, Li J, Tan N, Pan D, Zhang H, Xie L, Yao G, Shu H, Yao M, Wan D, Gu J, Yang S. The growth and metastasis of human hepatocellular carcinoma xenografts are inhibited by small interfering RNA targeting to the subunit ATP6L of proton pump. Cancer Res. 2005 Aug 1;65(15):6843-9 (Corresponding Author).
3、Xie L, Qin WX, Li JJ, He XH, Shu HQ, Yao GF, Wan DF, Gu JR. cDNA expression array analysis of gene expression in human hepatocarcinoma Hep3B cells induced by BNIPL-1. Acta Biochim Biophys Sin (Shanghai). 2005 Sep;37(9):618-24 (Corresponding Author).
4、Wan D, Gong Y, Qin W, Zhang P, Li J, Wei L, Zhou X, Li H, Qiu X, Zhong F, He L, Yu J, Yao G, Jiang H, Qian L, Yu Y, Shu H, Chen X, Xu H, Guo M, Pan Z, Chen Y, Ge C, Yang S, Gu J. Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15724-9 (Co-first author).
5、Xie L, Qin WX, He XH, Shu HQ, Yao GF, Wan DF, Gu JR l. Differential gene expression in human hepatocellular carcinoma Hep3B cells induced by apoptosis-related gene BNIPL-2. World J Gastroenterol. 2004 May 1;10(9):1286-91 (Corresponding Author).
6、Qin W, Hu J, Guo M, Xu J, Li J, Yao G, Zhou X, Jiang H, Zhang P, Shen L, Wan D, Gu J. BNIPL-2, a novel homologue of BNIP-2, interacts with Bcl-2 and Cdc42GAP in apoptosis. Biochem Biophys Res Commun. 2003 Aug 22;308(2):379-85.
7、Qin WX, Wan F, Sun FY, Zhang PP, Han LW, Huang Y, Jiang HQ, Zhao XT, He M, Ye Y, Cong WM, Wu MC, Zhang LS, Yang NW, Gu JR. Cloning and characterization of a novel gene (C17orf25) from the deletion region on chromosome 17p13.3 in hepatocelular carcinoma. Cell Res. 2001 Sep;11(3):209-16.
8、Pan H, Qin WX, Huo KK, Wan DF, Yu Y, Xu ZG, Hu QD, Gu KT, Zhou XM, Jiang HQ, Zhang PP, Huang Y, Li YY, Gu JR. Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1. Genomics. 2001 Sep;77(1-2):58-64 (Co-first author).
Review
1、Fais S, De Milito A, You H, Qin W. Targeting Vacuolar H+-ATPases as a New Strategy against Cancer. Cancer Res. 2007 Nov 15;67(22):10627-30 (Corresponding Author).
Lab Members
Fei Chen, Ph. D., Associate Professor. fchen@shsci.org, +86-21-34206022(T), +86-21-34206022(F)
Haiyan You, M. Sc., Instructor. hyou@shsci.org, +86-21-64436572(T), +86-21-64432142(F)
Genfu Yao, B. Sc., Associate Professor. gyao@shsci.org, +86-21-64436572(T), +86-21-64432142(F)
Huiqun Shu, B. Sc., Research Associate. hshu@shsci.org, +86-21-64436793(T), +86-21-64432142(F)
Graduate Students
Bin Yu, Ph. D. student, Fudan University Medical School. +86-21-64436572(T)
Jie Jin, Ph. D. student, Jiangsu University College of Life Sciences. +86-21-64436572(T)
Qin Cheng, Graduate student, Fudan University Medical School. +86-21-64436572(T)
Ning Tang, Graduate student, Fudan University Medical School. +86-21-64436572(T)
Ping Zhang, Graduate student, Shanghai Jiao Tong University School of Medicine. +86-21-34206022(T)
Wenxiang Kuang, Graduate student, Shanghai Jiao Tong University School of Medicine. +86-21-34206022(T)
Yun Deng, Graduate student, Shanghai Jiao Tong University School of Medicine. +86-21-64436572(T)
Qiujin Shen, Graduate student, Shanghai Jiao Tong University School of Medicine. +86-21-64436572(T) |
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